Understanding Effects of Ag and Fe3O4 Nanoparticles in Morpho-biochemical Variations of Seeds and its Localization

The primary focus of the current work was to synthesis Ag and Fe3O4 NPs through chemical routes and their varied concentration effect in the germination-seedling growth of Macrotyloma uniflorum (Kulthi bean) and Lens culinaris (Masoor) seeds, respectively. The synthesized nanoparticles were characterized by various physiochemical techniques (UV-Vis, DLS, FTIR, and FESEM). Four different concentrations of Ag and Fe3O4 NPs were applied on Lens culinaris and Macrotyloma uniflorum and positive influence was noticed for shoot-root length, chlorophyll, protein and starch content. Statistical analysis for every treatment was done with 3 replicates and results were analysed by one-way ANOVA using Minitab Version 16. Further, for getting information on the accumulation of nanoparticles in particular regions of plant body, ICP-OES study was also performed. The distinctive crystals of a black red colour in plant tissue was possible to identify the NPs localization in several seedling segments. Chemically synthesized metal-based nanoparticles have shown better effect on seed germination and have less hazardous impacts on the surrounding environment which make them a better alternative for commercially available agrochemicals.

Nanoparticles; Lens culinaris; Macrotyloma uniflorum; NPs localization

Basu, S., Sahu, N., Kumari, M., Mukherjee, K.  (2025): Understanding Effects of Ag and Fe3O4 Nanoparticles in Morpho-biochemical Variations of Seeds and its Localization . Scientia Agriculturae Bohemica, 56, 2, 6, DOI: 10.7160/sab.2025.560206

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