The primary focus of the current work was to synthesis Ag and Fe3O4 NPs through chemical routes and their varied concentration effect in the germination-seedling growth of Macrotyloma uniflorum (Kulthi bean) and Lens culinaris (Masoor) seeds, respectively. The synthesized nanoparticles were characterized by various physiochemical techniques (UV-Vis, DLS, FTIR, and FESEM). Four different concentrations of Ag and Fe3O4 NPs were applied on Lens culinaris and Macrotyloma uniflorum and positive influence was noticed for shoot-root length, chlorophyll, protein and starch content. Statistical analysis for every treatment was done with 3 replicates and results were analysed by one-way ANOVA using Minitab Version 16. Further, for getting information on the accumulation of nanoparticles in particular regions of plant body, ICP-OES study was also performed. The distinctive crystals of a black red colour in plant tissue was possible to identify the NPs localization in several seedling segments. Chemically synthesized metal-based nanoparticles have shown better effect on seed germination and have less hazardous impacts on the surrounding environment which make them a better alternative for commercially available agrochemicals.
Nanoparticles; Lens culinaris; Macrotyloma uniflorum; NPs localization
Basu, S., Sahu, N., Kumari, M., Mukherjee, K. (2025): Understanding Effects of Ag and Fe3O4 Nanoparticles in Morpho-biochemical Variations of Seeds and its Localization . Scientia Agriculturae Bohemica, 56, 2, 6, DOI: 10.7160/sab.2025.560206