Occurrence of Enterocin Genes in Enterococci from Slovak Milk Product Žinčica

Žinčica is a popular Slovak dairy product made from ewes’ milk. It is a by-product resulting during ewes’ lump cheese processing. Microbiota in Žinčica have rarely been studied, especially enterococci; however, they can produce beneficial substances – bacteriocins. In this study, the presence of Enterocins (Ents) genes were analysed in enterococci from Žinčica and partially also the inhibition activity. Samples of Žinčica were collected from different agrofarms producing ewes’ lump cheese (34) in Central Slovakia. In the enterococci tested, Ent P gene was the most frequently detected (in 6 out of 7 enterococci), followed by Ent A and Ent L50B genes. Ent B gene was detected only in E. faecium 30E1. On the other hand, E. faecalis 31E2 did not contain Ent genes, although it showed inhibition activity against the indicator strains Enterococcus avium EA5, Staphylococcus aureus SA5, Listeria monocytogenes CCM4699 (inhibition zone sizing up to 20 mm). E. faecium 30E1 contained genes of four Ents; however, it showed no inhibition activity. Growth of the four indicators was inhibited due to the antimicrobial activity of E. faecium 32E1 with Ent P gene detection. This is the first study reporting on the occurrence of Ent genes in enterococci from Žinčica.

Traditional milk product, enterococci, bacteriocin, inhibition

 Lauková, A., Strompfová, V., Tomáška, M., Kološta, M. (2019): Occurrence of Enterocin Genes in Enterococci from Slovak Milk Product Žinčica. Scientia Agriculturae Bohemica, 50, 197-202. DOI: 10.2478/sab-2019-0027

Files for download

Další články v rubrice

English ☰ Menu

We use cookies on the web presentations of the Czech University of Life Sciences Prague (under the czu.cz domain). These files give us ways to serve our services better and help us analyze site performance. We can share information about how you use our sites with our social media, advertising, and analytics partners. In the settings, you can choose which cookies we can use. You can change or revoke your consent at any time.